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https://genome.senckenberg.de/download/TOGA2/TOGA2integration/v1/Mammalia/Oryctolagus_cuniculus__Rabbit__HLoryCuni5__GCF_964237555.1/query_annotation.gff.gz diff --git a/TOGA2/manifest.yaml b/TOGA2/manifest.yaml new file mode 100644 index 0000000..688ee63 --- /dev/null +++ b/TOGA2/manifest.yaml @@ -0,0 +1,6 @@ +provider_name: "Hiller Lab, Senckenberg Research Institute" +pipeline_method: "TOGA2 comparative genome annotation pipeline" +pipeline_version: "2.0.9" +project_display_name: "TOGA2 vertebrate gene annotations" +description: "Comparative gene annotations generated with the TOGA2 pipeline. Different vertebrate clades were annotated using clade-specific reference assemblies for mammals, birds, testudines, and percomorph fishes. See the project homepage for details on reference assemblies and methodology." +homepage_url: "https://genome.senckenberg.de" diff --git a/scripts/generate_toga2_annotations.py b/scripts/generate_toga2_annotations.py new file mode 100755 index 0000000..d22f6c2 --- /dev/null +++ b/scripts/generate_toga2_annotations.py @@ -0,0 +1,119 @@ +#!/usr/bin/env python3 +"""Generate annotrieve-registry TOGA2/annotations.tsv from Senckenberg directory listings.""" + +from __future__ import annotations + +import argparse +import re +import sys +import urllib.request +from pathlib import Path + +TOGA2_BASE = "https://genome.senckenberg.de/download/TOGA2/TOGA2integration/v1" +TSV_HEADER = "assembly_accession\taccess_url" +ACCESSION_RE = re.compile(r"(GCA_\d+\.\d+|GCF_\d+\.\d+)") +FOLDER_LINK_RE = re.compile(r' str: + req = urllib.request.Request(url, headers={"User-Agent": "annotrieve-registry-generator/1.0"}) + with urllib.request.urlopen(req, timeout=120) as resp: + return resp.read().decode("utf-8", errors="replace") + + +def list_folders(index_url: str) -> list[str]: + html = fetch_index(index_url) + folders: list[str] = [] + seen: set[str] = set() + for match in FOLDER_LINK_RE.finditer(html): + href = match.group(1) + if href in ("../", "./") or href.startswith("/"): + continue + name = href.rstrip("/") + if name and name not in seen: + seen.add(name) + folders.append(name) + return folders + + +def extract_accession(folder_name: str) -> str | None: + match = ACCESSION_RE.search(folder_name) + return match.group(1) if match else None + + +def folder_has_gff(folder_url: str) -> bool: + html = fetch_index(folder_url) + return f'href="{GFF_FILE}"' in html or f">{GFF_FILE}<" in html + + +def build_rows(taxonomic_class: str, *, verify_gff: bool = True) -> list[tuple[str, str]]: + index_url = f"{TOGA2_BASE}/{taxonomic_class}/" + rows: list[tuple[str, str]] = [] + skipped: list[str] = [] + folders = list_folders(index_url) + for i, folder in enumerate(folders, start=1): + accession = extract_accession(folder) + if not accession: + continue + url = f"{index_url}{folder}/{GFF_FILE}" + if verify_gff: + folder_url = f"{index_url}{folder}/" + if not folder_has_gff(folder_url): + skipped.append(f"{accession} ({folder})") + continue + if i % 50 == 0: + print(f"verified {i}/{len(folders)} folders...", file=sys.stderr) + rows.append((accession, url)) + if skipped: + print(f"skipped {len(skipped)} folders without {GFF_FILE}:", file=sys.stderr) + for entry in skipped: + print(f" - {entry}", file=sys.stderr) + return rows + + +def write_tsv(path: Path, rows: list[tuple[str, str]]) -> None: + accessions = [acc for acc, _ in rows] + urls = [url for _, url in rows] + + dup_acc = sorted({a for a in accessions if accessions.count(a) > 1}) + dup_url = sorted({u for u in urls if urls.count(u) > 1}) + if dup_acc: + raise SystemExit(f"duplicate assembly_accession values: {dup_acc[:5]}") + if dup_url: + raise SystemExit(f"duplicate access_url values: {dup_url[:5]}") + + path.parent.mkdir(parents=True, exist_ok=True) + lines = [TSV_HEADER] + [f"{acc}\t{url}" for acc, url in rows] + path.write_text("\n".join(lines) + "\n", encoding="utf-8") + + +def main() -> None: + parser = argparse.ArgumentParser(description=__doc__) + parser.add_argument( + "--class", + dest="taxonomic_class", + default="Mammalia", + help="TOGA2 taxonomic class subdirectory (default: Mammalia)", + ) + parser.add_argument( + "--output", + type=Path, + default=Path("TOGA2/annotations.tsv"), + help="Output TSV path relative to repo root (default: TOGA2/annotations.tsv)", + ) + parser.add_argument( + "--no-verify-gff", + action="store_true", + help="Skip per-folder check that query_annotation.gff.gz exists", + ) + args = parser.parse_args() + + rows = build_rows(args.taxonomic_class, verify_gff=not args.no_verify_gff) + rows.sort(key=lambda row: row[0]) + write_tsv(args.output, rows) + print(f"Wrote {len(rows)} rows to {args.output}", file=sys.stderr) + + +if __name__ == "__main__": + main() diff --git a/scripts/report_toga2_missing_gff.py b/scripts/report_toga2_missing_gff.py new file mode 100755 index 0000000..d6a62e0 --- /dev/null +++ b/scripts/report_toga2_missing_gff.py @@ -0,0 +1,192 @@ +#!/usr/bin/env python3 +"""Report TOGA2 folders missing query_annotation.gff.gz across vertebrate clades.""" + +from __future__ import annotations + +import argparse +import re +import sys +import urllib.error +import urllib.request +from dataclasses import dataclass +from pathlib import Path + +TOGA2_BASE = "https://genome.senckenberg.de/download/TOGA2/TOGA2integration/v1" +DEFAULT_CLASSES = ("Mammalia", "Aves", "Percomorpha", "Testudines") +ACCESSION_RE = re.compile(r"(GCA_\d+\.\d+|GCF_\d+\.\d+)") +FOLDER_LINK_RE = re.compile(r' str | None: + req = urllib.request.Request(url, headers={"User-Agent": "annotrieve-registry-reporter/1.0"}) + try: + with urllib.request.urlopen(req, timeout=120) as resp: + return resp.read().decode("utf-8", errors="replace") + except urllib.error.HTTPError as exc: + if exc.code == 404: + return None + raise + + +def list_folders(index_url: str) -> list[str]: + html = fetch_index(index_url) + if html is None: + raise SystemExit(f"could not list folders at {index_url}") + folders: list[str] = [] + seen: set[str] = set() + for match in FOLDER_LINK_RE.finditer(html): + href = match.group(1) + if href in ("../", "./") or href.startswith("/"): + continue + name = href.rstrip("/") + if name and name not in seen: + seen.add(name) + folders.append(name) + return folders + + +def extract_accession(folder_name: str) -> str | None: + match = ACCESSION_RE.search(folder_name) + return match.group(1) if match else None + + +def folder_has_gff(folder_url: str) -> bool: + html = fetch_index(folder_url) + if html is None: + return False + return f'href="{GFF_FILE}"' in html or f">{GFF_FILE}<" in html + + +def scan_class(taxonomic_class: str) -> list[MissingGffFolder]: + index_url = f"{TOGA2_BASE}/{taxonomic_class}/" + missing: list[MissingGffFolder] = [] + folders = list_folders(index_url) + for i, folder in enumerate(folders, start=1): + folder_url = f"{index_url}{folder}/" + if folder_has_gff(folder_url): + continue + missing.append( + MissingGffFolder( + taxonomic_class=taxonomic_class, + folder_name=folder, + folder_url=folder_url, + assembly_accession=extract_accession(folder), + ) + ) + if i % 50 == 0: + print( + f"[{taxonomic_class}] checked {i}/{len(folders)} folders " + f"({len(missing)} missing {GFF_FILE})...", + file=sys.stderr, + ) + print( + f"[{taxonomic_class}] done: {len(folders)} folders, " + f"{len(missing)} missing {GFF_FILE}", + file=sys.stderr, + ) + return missing + + +def write_tsv(path: Path, entries: list[MissingGffFolder]) -> None: + path.parent.mkdir(parents=True, exist_ok=True) + lines = ["taxonomic_class\tfolder_name\tassembly_accession\tfolder_url"] + for entry in entries: + acc = entry.assembly_accession or "" + lines.append(f"{entry.taxonomic_class}\t{entry.folder_name}\t{acc}\t{entry.folder_url}") + path.write_text("\n".join(lines) + "\n", encoding="utf-8") + + +def write_markdown(path: Path, entries: list[MissingGffFolder], classes: tuple[str, ...]) -> None: + path.parent.mkdir(parents=True, exist_ok=True) + by_class: dict[str, list[MissingGffFolder]] = {cls: [] for cls in classes} + for entry in entries: + by_class.setdefault(entry.taxonomic_class, []).append(entry) + + with_accession = sum(1 for e in entries if e.assembly_accession) + without_accession = len(entries) - with_accession + + lines = [ + "# TOGA2 folders missing query_annotation.gff.gz", + "", + f"Base URL: [{TOGA2_BASE}/]({TOGA2_BASE}/)", + "", + "## Summary", + "", + "| Clade | Total folders missing GFF | With NCBI accession | Without accession |", + "|-------|---------------------------|---------------------|-------------------|", + ] + for cls in classes: + cls_entries = by_class.get(cls, []) + cls_with = sum(1 for e in cls_entries if e.assembly_accession) + lines.append( + f"| {cls} | {len(cls_entries)} | {cls_with} | {len(cls_entries) - cls_with} |" + ) + lines.extend( + [ + f"| **Total** | **{len(entries)}** | **{with_accession}** | **{without_accession}** |", + "", + "## Folders", + "", + ] + ) + + for cls in classes: + cls_entries = sorted(by_class.get(cls, []), key=lambda e: e.folder_name) + if not cls_entries: + continue + lines.append(f"### {cls}") + lines.append("") + for entry in cls_entries: + acc_suffix = f" (`{entry.assembly_accession}`)" if entry.assembly_accession else "" + lines.append(f"- [{entry.folder_name}]({entry.folder_url}){acc_suffix}") + lines.append("") + + path.write_text("\n".join(lines) + "\n", encoding="utf-8") + + +def main() -> None: + parser = argparse.ArgumentParser(description=__doc__) + parser.add_argument( + "--classes", + nargs="+", + default=list(DEFAULT_CLASSES), + help=f"TOGA2 clade subdirectories to scan (default: {' '.join(DEFAULT_CLASSES)})", + ) + parser.add_argument( + "--output-tsv", + type=Path, + default=Path("reports/toga2_missing_gff.tsv"), + help="Output TSV path (default: reports/toga2_missing_gff.tsv)", + ) + parser.add_argument( + "--output-md", + type=Path, + default=Path("reports/toga2_missing_gff.md"), + help="Output Markdown report path (default: reports/toga2_missing_gff.md)", + ) + args = parser.parse_args() + classes = tuple(args.classes) + + all_missing: list[MissingGffFolder] = [] + for taxonomic_class in classes: + all_missing.extend(scan_class(taxonomic_class)) + + all_missing.sort(key=lambda e: (e.taxonomic_class, e.folder_name)) + write_tsv(args.output_tsv, all_missing) + write_markdown(args.output_md, all_missing, classes) + + print(f"Wrote {len(all_missing)} rows to {args.output_tsv}", file=sys.stderr) + print(f"Wrote report to {args.output_md}", file=sys.stderr) + + +if __name__ == "__main__": + main()