Hi Warpers,
I am trying to pick particles from my recent Krios collection. To my knowledge, I am using the right gain reference file. I flipped the gain reference .mrc file in Y-axis, yet particles fall under DESELECTED category and appears grey (Image attached).
My dataset has 7000 tif files. I selected a random 40 tif files, moved to a separate folder along with the reference folder and flipped the reference file in X-axis. Fortunately, it works meaning Warp is picking up my particles and now they fall under PROCESSED category and appears green. However, when I do the same for the entire dataset of 7000 files, it FAILS AGAIN.
I am new to Warp, and I am unable to move ahead from here.
Any suggestions/advise in this regard will be highly appreciated!
Thanks,
Abhipsa
BioChemistry PhD student
City University of New York
Hi Warpers,
I am trying to pick particles from my recent Krios collection. To my knowledge, I am using the right gain reference file. I flipped the gain reference .mrc file in Y-axis, yet particles fall under DESELECTED category and appears grey (Image attached).
My dataset has 7000 tif files. I selected a random 40 tif files, moved to a separate folder along with the reference folder and flipped the reference file in X-axis. Fortunately, it works meaning Warp is picking up my particles and now they fall under PROCESSED category and appears green. However, when I do the same for the entire dataset of 7000 files, it FAILS AGAIN.
I am new to Warp, and I am unable to move ahead from here.
Any suggestions/advise in this regard will be highly appreciated!
Thanks,
Abhipsa
BioChemistry PhD student
City University of New York