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daniil-11-ger/README.md

Hi, I'm Daniil Gerassimov👋

Biotechnology Student | 4th Year (Dual Degree: University of Arizona & Kozybayev University)| Bioinformatics Enthusiast

I am a researcher and data analyst focused on the intersection of Plant Genomics and Digital Phenotyping. My work bridges the gap between raw biological data and actionable agricultural insights.

Technical Expertise:

  • Genomics / TE Annotation: Comparative Genomics, Transposable Element (TE) Annotation, PFM Motif Analysis, De novo assembly (Hifiasm, Jellyfish), RagTag scaffolding, Prokka, BLAST+, LTR retrotransposon analysis, RITE DB validation.
  • Data Science / Analytics: R (tidyverse, ggplot2, ANOVA, linear models), Python, Bash scripting, HPC (PBS/Slurm) workflows.
  • AI / Software Engineering: Model Context Protocol (MCP), LLM function calling, REST API design, Git/GitHub, Docker reproducibility.
  • Experimental / Wet Lab: CTAB DNA extraction, PCR, gel electrophoresis, microbial cultivation, spectrophotometry & bioassays.
  • Engineering: Laboratory automation (Python GUIs) and Cloud-based sample tracking (MIT App Inventor).

  • Experimental / Wet Lab: Recombinant protein production (Pichia pastoris expression), Gibson/NEBuilder Assembly, Tn5 transposon mutagenesis, CTAB & Miniprep DNA extraction, PCR/Colony-PCR, SDS-PAGE, Agarose Gel Electrophoresis, Spectrophotometry (NanoDrop).
  • Bioinformatics & In Silico Cloning: Geneious Prime (plasmid mapping, Nanopore/Plasmidsaurus verification), BLAST+, R/Bioconductor, Slurm HPC.

Experience:

  • University of Arizona (BIO5 / Wing Lab): Comparative genomics of transposable elements in wild rice.
  • MBZUAI (CBCRG Lab / UGRIP 2026): Ask JASPAR — LLM & MCP-driven regulatory genomics tools.
  • Kozybayev Labs: Environmental biotechnology & biosensors.
  • KAUST (Jesse Poland Lab): Research internship focusing on digital phenotyping and high-throughput field data analysis.

Featured Projects

Core Research / Computational Genomics

Oryza TE Dynamics: Bioinformatic pipeline and visualization tools for investigating Transposable Element dynamics in Oryza CC/BBCC genomes.

  • Focus: Bioinformatic pipeline and visualization tools for investigating Transposable Element (TE) dynamics across Oryza CC/BBCC genomes.
  • Tools: R, Python, Bash, HPC (UA Cluster), CyVerse.

AskJASPAR (MBZUAI CBCRG Lab — Private Repository):

  • Natural-language interface and MCP server tools for querying transcription factor binding profiles from JASPAR.
  • My Contribution: Designed the result-ranking layer, built pagination-aware retrieval (1,700+ records/query), engineered compare_matrices (Pearson correlation), and conducted benchmark parsing evaluations (doubling accuracy from 25% to ~54%).
  • Focus: Eukaryotic genome reconstruction using PacBio HiFi reads.
  • Tools: Hifiasm, Jellyfish, RagTag, Augustus.
  • Key Skill: Scaffolding, k-mer profiling, and organelle sorting.
  • Live Report: View Project Website
  • Focus: Automated workflow for E. coli genome assembly.
  • Tools: fastp, SPAdes, Prokka, QUAST.
  • Key Skill: Automation & Genomic Annotation.
  • Focus: Identifying significant genes in stress-response transcriptomes (LS vs HS).
  • Tools: R, Tidyverse, ggplot2.
  • Key Skill: Statistical hypothesis testing and biological visualization (Volcano Plots).

Analytics, Automation & Data Engineering

  • Focus: Automated summarization of multi-trait field breeding trials.
  • Tools: R, Functional Programming, dplyr.
  • Key Skill: Data engineering and automated plot-level variability analysis.
  • Focus: Reproducible R pipeline and interactive Shiny web dashboard for cleaning, QC filtering, and mixed-model analysis (LMM/BLUPs) of field trial data.
  • Tools: R (tidyverse, lme4), R Shiny, Docker, Field Book CSV.
  • Key Features: Automated dual-layer outlier detection (biological limits + IQR), spatial field layout visualization, and exportable summary tables for breeding research.
  • Focus: Python desktop tools for routine lab calculations.
  • Features: Tm calculation (Wallace Rule), DNA-to-RNA transcription.
  • Key Skill: Python Software Development & UI Design.
  • Focus: Cloud-based Android application for laboratory inventory.
  • Features: Real-time data sync via CloudDB, item management for reagents/samples.
  • Key Skill: Mobile Development (MIT App Inventor) & Cloud Integration.
  • Focus: Large-scale sequence identification and hit validation.
  • Tools: NCBI BLAST+, Bioconductor, R.
  • Key Skill: Processing command-line output into statistical reports.
  • Focus: Intensive curriculum for biological data analysis and inference.
  • Topics: CLT simulations, ANOVA, Tukey HSD, and Linear Regression.
  • Key Skill: Mathematical foundations and reproducible modeling.

Tech Stack

Python R Bash Linux Git Jupyter Docker Geneious Streamlit


Connect / Publications

Pinned Loading

  1. agronomic-data-automation-r agronomic-data-automation-r Public

    Automated data processing pipeline for multi-trait plant breeding trials. Uses functional programming in R to summarize plot-level statistics (Mean, SD, N) and analyze inter-plot variability

    R

  2. differential-gene-expression-R differential-gene-expression-R Public

    Gene expression profiling (LS vs HS samples) using Tidyverse. Includes paired t-tests, fold change calculation, and visualization via Volcano plots and Boxplots.

    R

  3. oryza-sativa-genome-assembly oryza-sativa-genome-assembly Public

    Advanced plant genome assembly pipeline for Oryza sativa using HiFi reads, k-mer profiling (Jellyfish), and Hifiasm

    HTML

  4. plant-phenomics-kaust plant-phenomics-kaust Public

    Field phenotyping data analysis from KAUST internship. Includes trait distribution, species comparison (t-test), and automated plot summarization in R.

    R

  5. r-statistics-foundations r-statistics-foundations Public

    A comprehensive 10-day intensive curriculum on Statistics and R for Biological Sciences. Covers data wrangling, probability distributions, hypothesis testing (t-test, ANOVA), and linear regression

    R

  6. sequence-homology-search-blast sequence-homology-search-blast Public

    Bioinformatics pipeline for sequence identification using NCBI BLAST+. Includes command-line data extraction and R-based statistical filtering of genomic hits

    R